Sperm DNA Fragmentation, Determined Using the Sperm Chromatin Dispersion (SCD) Test, A Study in Republic of Kosovo Population

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The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation.

Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation in semen. The SCD test is based on the principle that sperm with fragmented DNA fail to produce the characteristic halo of dispersed DNA loops that is observed in sperm with non-fragmented DNA, foll...

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Effects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm

Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmen...

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effects of sperm dna fragmentation on semen parameters and icsi outcome determined by an improved scd test, halosperm

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

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Assessment of Sperm Deoxyribose Nucleic Acid Fragmentation Using Sperm Chromatin Dispersion Assay

The integrity of sperm deoxyribose nucleic acid (DNA) is one of the determinants that ensure normal fertilization, implantation, pregnancy, and the generation of a healthy progeny. Assessment of sperm DNA fragmentation has gained importance as a tool to provide significant information regarding sperm quality, and it can independently predict sperm fertilizing potential. The sperm chromatin disp...

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Simultaneous observation of DNA fragmentation and protein loss in the boar spermatozoon following application of the sperm chromatin dispersion (SCD) test.

DNA fragmentation and the nuclear protein matrix in boar spermatozoa were simultaneously assessed using a specific variant of the sperm chromatin dispersion (SCD) test that allows direct visualization of DNA and nuclear proteins under standard conditions of chemical lysis. Nuclear proteins remaining after lysis were stained with the fluorochrome 2,7-dibrom-4-hydroxy-mercury-fluorescein for spec...

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ژورنال

عنوان ژورنال: International Journal of Biology

سال: 2018

ISSN: 1916-968X,1916-9671

DOI: 10.5539/ijb.v10n3p14